Phycoerythrin (PE)

The excitation spectrum and emission spectra (bold line) are shown for PE. The red-shaded region represents the bandpass filter commonly used to measure PE fluorescence. On most flow cytometers, PE is excited by an argon laser tuned to 488 nm; however, much more sensitive detection is obtained with a laser tuned to 533 nm.

Also see the absorbance spectrum for PE.

Compare the PE fluorescence spectra to spectra for several FACS immunofluorescence dyes.